Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer

Christian F. Gainer; Marek Romanowski

doi:10.1117/12.2002551

22 February 2013 Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer

Christian F. Gainer, Marek Romanowski

Proceedings Volume 8595, Colloidal Nanocrystals for Biomedical Applications VIII; 85950O (2013) https://doi.org/10.1117/12.2002551
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

While upconverting lanthanide nanoparticles have numerous advantages over other exogenous contrast agents used in scanned multiphoton imaging, their long luminescence lifetimes cause images collected with non-descanned detection to be greatly blurred. We demonstrate herein the use of Richardson-Lucy deconvolution to deblur luminescence images obtained via multiphoton scanning microscopy. Images were taken of three dimensional models of colon and ovarian cancer following incubation with NaYF₄:Yb,Er nanoparticles functionalized with an antibody for EGFR and folic acid respectively. Following deconvolution, images had a lateral resolution on par with the optimal performance of the imaging system used, ~1.2 μm, and an axial resolution of ~5 μm. Due to the relatively high multiphoton excitation efficiency of these nanoparticles, it is possible to follow binding of individual particles in tissue. In addition, their extreme photostability allows for prolonged imaging without significant loss in luminescence signal. With these advantageous properties in mind, we also discuss the potential application of upconverting lanthanide nanoparticles for tracking of specific, cancer relevant receptors in tissue.

Citation Download Citation

Christian F. Gainer and Marek Romanowski "Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer", Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 85950O (22 February 2013); https://doi.org/10.1117/12.2002551

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KEYWORDS

Nanoparticles

Luminescence

Lanthanides

3D modeling

Colon

Tissues

Coating

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