Stationary microfluidics: molecular diagnostic assays by moving magnetic beads through non-moving liquids

Holger Becker; Cornelia Carstens; Dirk Kuhlmeier; Natalia Sandetskaya; Nicole Schröter; Christian Zilch; Claudia Gärtner

doi:10.1117/12.2004041

13 March 2013 Stationary microfluidics: molecular diagnostic assays by moving magnetic beads through non-moving liquids

Holger Becker, Cornelia Carstens, Dirk Kuhlmeier, Natalia Sandetskaya, Nicole Schröter, Christian Zilch, Claudia Gärtner

Author Affiliations +

Proceedings Volume 8615, Microfluidics, BioMEMS, and Medical Microsystems XI; 86150B (2013) https://doi.org/10.1117/12.2004041
Event: SPIE MOEMS-MEMS, 2013, San Francisco, California, United States

Abstract

Commonly, microfluidic devices are based on the movement of fluids. For molecular diagnostics assays which often include steps like PCR, this practically always involves a more or less complicated set of external pumps, valves and liquid controls. In the presented paper, we follow a different approach in which the fluid after sample introduction remains stationary and the main bioactive sample molecules are moved through a chain of reaction compartments which contain the different reagents necessary for the assay. The big advantage of this concept is the lack of any external fluid actuation/control. Results on sample carry-over experiments and complete assays will be given.

Citation Download Citation

Holger Becker, Cornelia Carstens, Dirk Kuhlmeier, Natalia Sandetskaya, Nicole Schröter, Christian Zilch, and Claudia Gärtner "Stationary microfluidics: molecular diagnostic assays by moving magnetic beads through non-moving liquids ", Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 86150B (13 March 2013); https://doi.org/10.1117/12.2004041

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