Bioluminescence tomography improves quantitative accuracy for pre-clinical imaging

James A. Guggenheim; Hector R. A. Basevi; Iain B. Styles; Jon Frampton; Hamid Dehghani

doi:10.1117/12.2033317

14 June 2013 Bioluminescence tomography improves quantitative accuracy for pre-clinical imaging

James A. Guggenheim, Hector R. A. Basevi, Iain B. Styles, Jon Frampton, Hamid Dehghani

Proceedings Volume 8799, Diffuse Optical Imaging IV; 87990G (2013) https://doi.org/10.1117/12.2033317
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

A study is presented that demonstrates that bioluminescence tomography can reconstruct accurate 3D images of internal light sources placed at a range of depths within a physical phantom and that it provides more reliable quantitative data than standard bioluminescence imaging. Specifically, it is shown that when imaging sources at depths ranging from 5 to 15mm, estimates of total source strength are stable to within ±11% using tomography whilst values deduced by traditional methods vary 10-fold. Additionally, the tomographic approach correctly localises sources to within 1.5mm error in all cases considered.

Citation Download Citation

James A. Guggenheim, Hector R. A. Basevi, Iain B. Styles, Jon Frampton, and Hamid Dehghani "Bioluminescence tomography improves quantitative accuracy for pre-clinical imaging", Proc. SPIE 8799, Diffuse Optical Imaging IV, 87990G (14 June 2013); https://doi.org/10.1117/12.2033317

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