Mesoscopic tomography imaging of reporter genes in thick printed tissue constructs

Mehmet S. Ozturk; Vivian K. Lee; Lingling Zhao; Guohoa Dai; Xavier Intes

doi:10.1117/12.2032539

14 June 2013 Mesoscopic tomography imaging of reporter genes in thick printed tissue constructs

Mehmet S. Ozturk, Vivian K. Lee, Lingling Zhao, Guohoa Dai, Xavier Intes

Proceedings Volume 8799, Diffuse Optical Imaging IV; 87990Y (2013) https://doi.org/10.1117/12.2032539
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

We report an application of Mesoscopic Fluorescence Molecular Tomography to 3D tissue engineering construct. Engineered thick tissue was hosting two 3D printed vasculatures. The channels were formed by live cells, expressing GFP and mCherry reporter genes, embedded in 3mm turbid media. Tissue and cells kept in a 3mm thick perfusion chamber during the entire imaging process which took less than 5 minutes.

Citation Download Citation

Mehmet S. Ozturk, Vivian K. Lee, Lingling Zhao, Guohoa Dai, and Xavier Intes "Mesoscopic tomography imaging of reporter genes in thick printed tissue constructs", Proc. SPIE 8799, Diffuse Optical Imaging IV, 87990Y (14 June 2013); https://doi.org/10.1117/12.2032539

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