Experimental analysis of focal fields in laser scanning fluorescence stereomicroscopy

Yanlong Yang; Tong Peng; Ming Lei; Runze Li; Xing Zhou; Di Wu; Baoli Yao; Tong Ye

doi:10.1117/12.2040248

12 March 2014 Experimental analysis of focal fields in laser scanning fluorescence stereomicroscopy

Yanlong Yang, Tong Peng, Ming Lei, Runze Li, Xing Zhou, Di Wu, Baoli Yao, Tong Ye

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 89491R (2014) https://doi.org/10.1117/12.2040248
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Bessel beams have been proved to have the ability to extend the depth of focus in fluorescence microscopy. But the depth discrimination was not investigated thoroughly. Following our previous work², we investigated focal fields of Bessel-Gauss beams at different scanning angles. We found that the central focusing lines were tilted differently at different scanning angles. This effect manifests the ability of the true perspective view in the fluorescence stereomicroscopy.

Citation Download Citation

Yanlong Yang, Tong Peng, Ming Lei, Runze Li, Xing Zhou, Di Wu, Baoli Yao, and Tong Ye "Experimental analysis of focal fields in laser scanning fluorescence stereomicroscopy ", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 89491R (12 March 2014); https://doi.org/10.1117/12.2040248

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