Reflection-type long period grating biosensor for the detection of drug resistant bacteria: The Opto-bacteria Project

M. Consales; G. Quero; S. Zuppolini; L. Sansone; A. Borriello; M. Giordano; A. Venturelli; A. Cusano

doi:10.1117/12.2059783

2 June 2014 Reflection-type long period grating biosensor for the detection of drug resistant bacteria: The Opto-bacteria Project

M. Consales, G. Quero, S. Zuppolini, L. Sansone, A. Borriello, M. Giordano, A. Venturelli, A. Cusano

Author Affiliations +

Proceedings Volume 9157, 23rd International Conference on Optical Fibre Sensors; 91575G (2014) https://doi.org/10.1117/12.2059783
Event: OFS2014 23rd International Conference on Optical Fiber Sensors, 2014, Santander, Spain

Abstract

We report on the development of a multilayer coated reflection-type long period fiber grating (LPG) biosensor, useful for the detection of antibiotic resistance bacteria. A standard LPG is first transformed in a more practical probe working in reflection mode, then it is coated by a primary layer of aPS and a secondary layer of PMMA in order to increase its surrounding refractive index sensitivity and at the same time provide the necessary conditions for a correct biofunctionalization. Standard linkage chemistry has been applied to anchor the bioreceptors on the probe surface. We show some preliminary results demonstrating the capability of our LPG biosensor to successfully monitor all the biological steps of the biomolecular experiments, including β-lactamase binding detection tests.

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M. Consales, G. Quero, S. Zuppolini, L. Sansone, A. Borriello, M. Giordano, A. Venturelli, and A. Cusano "Reflection-type long period grating biosensor for the detection of drug resistant bacteria: The Opto-bacteria Project", Proc. SPIE 9157, 23rd International Conference on Optical Fibre Sensors, 91575G (2 June 2014); https://doi.org/10.1117/12.2059783

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