Visualization and neuronal cell targeting during electrophysiological recordings facilitated by quantum dots

Lauren D. Field; Bertalan K. Andrásfalvy; Gregorio L. Galiñanes; Daniel Huber; Mladen Barbic; John J. Macklin; Kimihiro Susumu; James B. Delehanty III; Alan L. Huston; Judit K. Makara; Igor L. Medintz

doi:10.1117/12.2076934

10 March 2015 Visualization and neuronal cell targeting during electrophysiological recordings facilitated by quantum dots

Lauren D. Field, Bertalan K. Andrásfalvy, Gregorio L. Galiñanes, Daniel Huber, Mladen Barbic, John J. Macklin, Kimihiro Susumu, James B. Delehanty III, Alan L. Huston, Judit K. Makara, Igor L. Medintz

Author Affiliations +

Proceedings Volume 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II; 93050Y (2015) https://doi.org/10.1117/12.2076934
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The simultaneous visualization, identification and targeting of neurons during patch clamp-mediated electrophysiological recordings is a basic technique in neuroscience, yet it is often complicated by the inability to visualize the pipette tip, particularly in deep brain tissue. Here we demonstrate a novel approach in which fluorescent quantum dot probes are used to coat pipettes prior to their use. The strong two-photon absorption cross sections of the quantum dots afford robust contrast at significantly deeper penetration depths than current methods allow. We demonstrate the utility of this technique in multiple recording formats both in vitro and in vivo where imaging of the pipettes is achieved at remarkable depths (up to 800 microns). Notably, minimal perturbation of cellular physiology is observed over the hours-long time course of neuronal recordings. We discuss our results within the context of the role that quantum dot nanoprobes may play in understanding neuronal cell physiology.

Citation Download Citation

Lauren D. Field, Bertalan K. Andrásfalvy, Gregorio L. Galiñanes, Daniel Huber, Mladen Barbic, John J. Macklin, Kimihiro Susumu, James B. Delehanty III, Alan L. Huston, Judit K. Makara, and Igor L. Medintz "Visualization and neuronal cell targeting during electrophysiological recordings facilitated by quantum dots", Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 93050Y (10 March 2015); https://doi.org/10.1117/12.2076934

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Neurons

Visualization

Quantum dots

Plasma

Tissues

Brain

Coating

Show All Keywords

Keywords/Phrases

Search In:

Publication Years