In vivo outcome study of BPD-mediated PDT using a macroscopic singlet oxygen model

Michele M. Kim; Rozhin Penjweini; Timothy C. Zhu

doi:10.1117/12.2077803

2 March 2015 In vivo outcome study of BPD-mediated PDT using a macroscopic singlet oxygen model

Michele M. Kim, Rozhin Penjweini, Timothy C. Zhu

Proceedings Volume 9308, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIV; 93080A (2015) https://doi.org/10.1117/12.2077803
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Macroscopic modeling of the apparent reacted singlet oxygen concentration ([¹O₂]_rx) for use with photodynamic therapy (PDT) has been developed and studied for benzoporphryin derivative monoacid ring A (BPD), a common photosensitizer. The four photophysical parameters (ξ, σ, β, δ) and threshold singlet oxygen dose ([¹O₂]_{rx, sh}) have been investigated and determined using the RIF model of murine fibrosarcomas and interstitial treatment delivery. These parameters are examined and verified further by monitoring tumor growth post-PDT. BPD was administered at 1 mg/kg, and mice were treated 3 hours later with fluence rates ranging between 75 – 150 mW/cm² and total fluences of 100 – 350 J/cm². Treatment was delivered superficially using a collimated beam. Changes in tumor volume were tracked following treatment. The tumor growth rate was fitted for each treatment condition group and compared using dose metrics including total light dose, PDT dose, and reacted singlet oxygen. Initial data showing the correlation between outcomes and various dose metrics indicate that reacted singlet oxygen serves as a good dosimetric quantity for predicting PDT outcome.

Citation Download Citation

Michele M. Kim, Rozhin Penjweini, and Timothy C. Zhu "In vivo outcome study of BPD-mediated PDT using a macroscopic singlet oxygen model", Proc. SPIE 9308, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIV, 93080A (2 March 2015); https://doi.org/10.1117/12.2077803

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