Radiometric calibration to consider in quantitative clinical fluorescence imaging measurements

M. Litorja; A. Urbas; Y. Zong

doi:10.1117/12.2086838

4 March 2015 Radiometric calibration to consider in quantitative clinical fluorescence imaging measurements

M. Litorja, A. Urbas, Y. Zong

Proceedings Volume 9311, Molecular-Guided Surgery: Molecules, Devices, and Applications; 931114 (2015) https://doi.org/10.1117/12.2086838
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The fluorescent light detected by a clinical imager is assumed to be proportional only to the amount of fluorescent substance present in the sample and the level of excitation. Unfortunately, there are many factors that can add or subtract to the light signal directly attributable to the desired fluorescence emission, especially with fluorescence from inside the body imaged remotely. The quantification of fluorescence emission is feasible by calibrating the imager using international system of units (SI)-traceable physical and material calibration artifacts such that the detector’s digital numbers (DN) can be converted to radiometric units. Here we discuss three calibration methods for quantitative clinical fluorescence imaging systems.

Citation Download Citation

M. Litorja, A. Urbas, and Y. Zong "Radiometric calibration to consider in quantitative clinical fluorescence imaging measurements", Proc. SPIE 9311, Molecular-Guided Surgery: Molecules, Devices, and Applications, 931114 (4 March 2015); https://doi.org/10.1117/12.2086838

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Imaging systems

Calibration

Sensors

Signal detection

Standards development

Molecules

Show All Keywords

Keywords/Phrases

Search In:

Publication Years