Optimisation of acquisition time in bioluminescence imaging

Shelley L. Taylor; Suzannah K. G. Mason; Sophie Glinton; Mark Cobbold; Iain B. Styles; Hamid Dehghani

doi:10.1117/12.2075661

5 March 2015 Optimisation of acquisition time in bioluminescence imaging

Shelley L. Taylor, Suzannah K. G. Mason, Sophie Glinton, Mark Cobbold, Iain B. Styles, Hamid Dehghani

Proceedings Volume 9319, Optical Tomography and Spectroscopy of Tissue XI; 93191T (2015) https://doi.org/10.1117/12.2075661
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Decreasing the acquisition time in bioluminescence imaging (BLI) and bioluminescence tomography (BLT) will enable animals to be imaged within the window of stable emission of the bioluminescent source, a higher imaging throughput and minimisation of the time which an animal is anaesthetised. This work investigates, through simulation using a heterogeneous mouse model, two methods of decreasing acquisition time: 1. Imaging at fewer wavelengths (a reduction from five to three); and 2. Increasing the bandwidth of filters used for imaging. The results indicate that both methods are viable ways of decreasing the acquisition time without a loss in quantitative accuracy. Importantly, when choosing imaging wavelengths, the spectral attenuation of tissue and emission spectrum of the source must be considered, in order to choose wavelengths at which a high signal can be achieved. Additionally, when increasing the bandwidth of the filters used for imaging, the bandwidth must be accounted for in the reconstruction algorithm.

Citation Download Citation

Shelley L. Taylor, Suzannah K. G. Mason, Sophie Glinton, Mark Cobbold, Iain B. Styles, and Hamid Dehghani "Optimisation of acquisition time in bioluminescence imaging", Proc. SPIE 9319, Optical Tomography and Spectroscopy of Tissue XI, 93191T (5 March 2015); https://doi.org/10.1117/12.2075661

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