Microfluidic system for the identification of bacterial pathogens causing urinary tract infections

Holger Becker; Nadine Hlawatsch; Tommy Haraldsson; Wouter van der Wijngaart; Anders Lind; Surbi Malhotra-Kumar; Agata Turlej-Rogacka; Herman Goossens

doi:10.1117/12.2077644

13 March 2015 Microfluidic system for the identification of bacterial pathogens causing urinary tract infections

Holger Becker, Nadine Hlawatsch, Tommy Haraldsson, Wouter van der Wijngaart, Anders Lind, Surbi Malhotra-Kumar, Agata Turlej-Rogacka, Herman Goossens

Author Affiliations +

Proceedings Volume 9320, Microfluidics, BioMEMS, and Medical Microsystems XIII; 93200S (2015) https://doi.org/10.1117/12.2077644
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Urinary tract infections (UTIs) are among the most common bacterial infections and pose a significant healthcare burden. The growing trend in antibiotic resistance makes it mandatory to develop diagnostic kits which allow not only the determination of a pathogen but also the antibiotic resistances. We have developed a microfluidic cartridge which takes a direct urine sample, extracts the DNA, performs an amplification using batch-PCR and flows the sample over a microarray which is printed into a microchannel for fluorescence detection. The cartridge is injection-molded out of COP and contains a set of two-component injection-molded rotary valves to switch between input and to isolate the PCR chamber during thermocycling. The hybridization probes were spotted directly onto a functionalized section of the outlet microchannel. We have been able to successfully perform PCR of E.coli in urine in this chip and perform a fluorescence detection of PCR products. An upgraded design of the cartridge contains the buffers and reagents in blisters stored on the chip.

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Holger Becker, Nadine Hlawatsch, Tommy Haraldsson, Wouter van der Wijngaart, Anders Lind, Surbi Malhotra-Kumar, Agata Turlej-Rogacka, and Herman Goossens "Microfluidic system for the identification of bacterial pathogens causing urinary tract infections", Proc. SPIE 9320, Microfluidics, BioMEMS, and Medical Microsystems XIII, 93200S (13 March 2015); https://doi.org/10.1117/12.2077644

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