Four-wave mixing microscopy: a high potential nonlinear imaging method

Tobias Ehmke; Andreas Knebl; Alexander Heisterkamp

doi:10.1117/12.2076743

5 March 2015 Four-wave mixing microscopy: a high potential nonlinear imaging method

Tobias Ehmke, Andreas Knebl, Alexander Heisterkamp

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 932912 (2015) https://doi.org/10.1117/12.2076743
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

In this work we present non-resonant four-wave mixing microscopy as an additional contrast mechanism in nonlinear microscopy. The setup for this technique was based on a commercially available multiphoton microscope setup equipped with a titanium:sapphire-laser and an optical parametric oscillator as light sources. Fundamental system characteristics with respect to the spatio-temporal pulse overlap and the influence of aberrations on the process are presented. Experiments regarding the directionality of the four-wave mixing signal performed on fresh porcine meat showed an average ratio of the backward to forward signal mean intensity of 0.16 ± 0.01. Nevertheless, structural information is comparable for both detection modalities. This highlights the potential of four-wave mixing microscopy for in vivo applications. Furthermore, results on porcine meat show the additional contrast generated by four-wave mixing. In summary, the results show a great potential of non-resonant four-wave mixing microscopy as label-free imaging modality in the biomedical sciences.

Citation Download Citation

Tobias Ehmke, Andreas Knebl, and Alexander Heisterkamp "Four-wave mixing microscopy: a high potential nonlinear imaging method", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932912 (5 March 2015); https://doi.org/10.1117/12.2076743

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available