Three dimensional time-gated tracking of non-blinking quantum dots in live cells

Matthew S. DeVore; Dominik G. Stich; Aaron M. Keller; Yagna Ghosh; Peter M. Goodwin; Mary E. Phipps; Michael H. Stewart; Cédric Cleyrat; Bridget S. Wilson; Diane S. Lidke; Jennifer A. Hollingsworth; James H. Werner

doi:10.1117/12.2082943

12 March 2015 Three dimensional time-gated tracking of non-blinking quantum dots in live cells

Matthew S. DeVore, Dominik G. Stich, Aaron M. Keller, Yagna Ghosh, Peter M. Goodwin, Mary E. Phipps, Michael H. Stewart, Cédric Cleyrat, Bridget S. Wilson, Diane S. Lidke, Jennifer A. Hollingsworth, James H. Werner

Author Affiliations +

Proceedings Volume 9338, Colloidal Nanoparticles for Biomedical Applications X; 933812 (2015) https://doi.org/10.1117/12.2082943
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. Signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.

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Matthew S. DeVore, Dominik G. Stich, Aaron M. Keller, Yagna Ghosh, Peter M. Goodwin, Mary E. Phipps, Michael H. Stewart, Cédric Cleyrat, Bridget S. Wilson, Diane S. Lidke, Jennifer A. Hollingsworth, and James H. Werner "Three dimensional time-gated tracking of non-blinking quantum dots in live cells", Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 933812 (12 March 2015); https://doi.org/10.1117/12.2082943

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