Protein-protein binding before and after photo-modification of albumin

Sarah C. Rozinek; Randolph D. Glickman; Robert J. Thomas; Lorenzo Brancaleon

doi:10.1117/12.2213632

7 March 2016 Protein-protein binding before and after photo-modification of albumin

Sarah C. Rozinek, Randolph D. Glickman, Robert J. Thomas, Lorenzo Brancaleon

Proceedings Volume 9706, Optical Interactions with Tissue and Cells XXVII; 97061J (2016) https://doi.org/10.1117/12.2213632
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Bioeffects of directed-optical-energy encompass a wide range of applications. One aspect of photochemical interactions involves irradiating a photosensitizer with visible light in order to induce protein unfolding and consequent changes in function. In the past, irradiation of several dye-protein combinations has revealed effects on protein structure. Beta lactoglobulin, human serum albumin (HSA) and tubulin have all been photo-modified with meso-tetrakis(4- sulfonatophenyl)porphyrin (TSPP) bound, but only in the case of tubulin has binding caused a verified loss of biological function (loss of ability to form microtubules) as a result of this light-induced structural change. The current work questions if the photo-induced structural changes that occur to HSA, are sufficient to disable its biological function of binding to osteonectin. The albumin-binding protein, osteonectin, is about half the molecular weight of HSA, so the two proteins and their bound product can be separated and quantified by size exclusion high performance liquid chromatography. TSPP was first bound to HSA and irradiated, photo-modifying the structure of HSA. Then native HSA or photo-modified HSA (both with TSPP bound) were compared, to assess loss in HSA’s innate binding ability as a result of light-induced structure modification.

Citation Download Citation

Sarah C. Rozinek, Randolph D. Glickman, Robert J. Thomas, and Lorenzo Brancaleon "Protein-protein binding before and after photo-modification of albumin", Proc. SPIE 9706, Optical Interactions with Tissue and Cells XXVII, 97061J (7 March 2016); https://doi.org/10.1117/12.2213632

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