Point spread function engineering with multiphoton SPIFI

Keith A. Wernsing; Jeffrey J. Field; Scott R. Domingue; Alyssa M. Allende-Motz; Keith F. DeLuca; Dean H. Levi; Jennifer G. DeLuca; Michael D. Young; Jeff A. Squier; Randy A. Bartels

doi:10.1117/12.2213090

9 March 2016 Point spread function engineering with multiphoton SPIFI

Keith A. Wernsing, Jeffrey J. Field, Scott R. Domingue, Alyssa M. Allende-Motz, Keith F. DeLuca, Dean H. Levi, Jennifer G. DeLuca, Michael D. Young, Jeff A. Squier, Randy A. Bartels

Author Affiliations +

Proceedings Volume 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII; 971304 (2016) https://doi.org/10.1117/12.2213090
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

MultiPhoton SPatIal Frequency modulated Imaging (MP-SPIFI) has recently demonstrated the ability to simultaneously obtain super-resolved images in both coherent and incoherent scattering processes — namely, second harmonic generation and two-photon fluorescence, respectively.¹ In our previous analysis, we considered image formation produced by the zero and first diffracted orders from the SPIFI modulator. However, the modulator is a binary amplitude mask, and therefore produces multiple diffracted orders. In this work, we extend our analysis to image formation in the presence of higher diffracted orders. We find that tuning the mask duty cycle offers a measure of control over the shape of super-resolved point spread functions in an MP-SPIFI microscope.

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Keith A. Wernsing, Jeffrey J. Field, Scott R. Domingue, Alyssa M. Allende-Motz, Keith F. DeLuca, Dean H. Levi, Jennifer G. DeLuca, Michael D. Young, Jeff A. Squier, and Randy A. Bartels "Point spread function engineering with multiphoton SPIFI", Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971304 (9 March 2016); https://doi.org/10.1117/12.2213090

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