Volumetric real-time wide-field microscopy with tunable acoustic gradient lens
(Conference Presentation)

Ting Hsuan Chen; Craig B. Arnold

doi:10.1117/12.2212604

27 April 2016 Volumetric real-time wide-field microscopy with tunable acoustic gradient lens (Conference Presentation)

Ting Hsuan Chen, Craig B. Arnold

Proceedings Volume 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII; 971307 (2016) https://doi.org/10.1117/12.2212604
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Three-dimensional real-time imaging is a fundamental challenge for understanding dynamic processes. In this presentation, we propose a new method based on a tunable acoustic gradient lens integrated in a simple optical system. By synchronizing a pulsed LED with a high-speed camera, we are able to resolve a volume of 2 millimeters and 2 millimeters with depth 1 millimeter in 7 microseconds. A simulation model of the optical system is provided and serves as a useful tool for designing the optical system for the desired aspect-ratio of the imaging volume. The ability to resolve a volume in microseconds opens the door to exploring the fundamental dynamics in micro scale.

Conference Presentation

Citation Download Citation

Ting Hsuan Chen and Craig B. Arnold "Volumetric real-time wide-field microscopy with tunable acoustic gradient lens (Conference Presentation)", Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971307 (27 April 2016); https://doi.org/10.1117/12.2212604

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KEYWORDS

Acoustics

Microscopy

Optical design

3D image processing

3D acquisition

High speed cameras

Imaging systems

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