Digital aberration correction of fluorescent images with coherent holographic image reconstruction by phase transfer (CHIRPT)

Jeffrey J. Field; Randy A. Bartels

doi:10.1117/12.2213526

9 March 2016 Digital aberration correction of fluorescent images with coherent holographic image reconstruction by phase transfer (CHIRPT)

Jeffrey J. Field, Randy A. Bartels

Author Affiliations +

Proceedings Volume 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII; 97130B (2016) https://doi.org/10.1117/12.2213526
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Coherent holographic image reconstruction by phase transfer (CHIRPT) is an imaging method that permits digital holographic propagation of fluorescent light. The image formation process in CHIRPT is based on illuminating the specimen with a precisely controlled spatio-temporally varying intensity pattern. This pattern is formed by focusing a spatially coherent illumination beam to a line focus on a spinning modulation mask, and image relaying the mask plane to the focal plane of an objective lens. Deviations from the designed spatio-temporal illumination pattern due to imperfect mounting of the circular modulation mask onto the rotation motor induce aberrations in the recovered image. Here we show that these aberrations can be measured and removed non-iteratively by measuring the disk aberration phase externally. We also demonstrate measurement and correction of systematic optical aberrations in the CHIRPT microscope.

Conference Presentation

Citation Download Citation

Jeffrey J. Field and Randy A. Bartels "Digital aberration correction of fluorescent images with coherent holographic image reconstruction by phase transfer (CHIRPT)", Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 97130B (9 March 2016); https://doi.org/10.1117/12.2213526

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