Comparison of Raman spectroscopy equipment for tissues and biofluids analysis

Yu. A. Khristoforova; I. A. Bratchenko; O. O. Myakinin; D. N. Artemyev; V. P. Zakharov

doi:10.1117/12.2227878

27 April 2016 Comparison of Raman spectroscopy equipment for tissues and biofluids analysis

Yu. A. Khristoforova, I. A. Bratchenko, O. O. Myakinin, D. N. Artemyev, V. P. Zakharov

Proceedings Volume 9887, Biophotonics: Photonic Solutions for Better Health Care V; 98873E (2016) https://doi.org/10.1117/12.2227878
Event: SPIE Photonics Europe, 2016, Brussels, Belgium

Abstract

In this study we demonstrate a comparative analysis of blood serum and normal human skin by Raman spectroscopy with application of different spectroscopic equipment. For serum analysis we measure a total concentration of proteins and compared it with intensity of 1002 cm^-1 Raman peak. Standard deviation for protein control in blood serum differed from 7.4% to 19% for different spectroscopic setups. For human skin control we used three Raman peaks near 1340, 1450 and 1650 cm^-1. Measurements of different skin samples were analyzed on the phase plane to find areas corresponding to the normal skin. Taking into account the different sensitivities of the detected signal with different detectors in the spectral range 810-950 nm we calculated correction coefficients allowed for making comparison of spectral measurements made on different spectrometers with ranging not exceeding 21%.

Citation Download Citation

Yu. A. Khristoforova, I. A. Bratchenko, O. O. Myakinin, D. N. Artemyev, and V. P. Zakharov "Comparison of Raman spectroscopy equipment for tissues and biofluids analysis", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98873E (27 April 2016); https://doi.org/10.1117/12.2227878

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available