Development of forward-scattered Raman tomography for monitoring treatment of rheumatoid arthritis in rodents

Elzbieta Stepula; Anders R. Walther; Christine K. Dam; Magnus Jensen; Martin A. B. Hedegaard; Mads S. Bergholt

doi:10.1117/12.2609556

7 March 2022 Development of forward-scattered Raman tomography for monitoring treatment of rheumatoid arthritis in rodents

Elzbieta Stepula, Anders R. Walther, Christine K. Dam, Magnus Jensen, Martin A. B. Hedegaard, Mads S. Bergholt

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Proceedings Volume PC11952, Multimodal Biomedical Imaging XVII; PC119520B (2022) https://doi.org/10.1117/12.2609556
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We have developed a forward-scattered high-resolution Raman-Computed Tomography (R-CT) technique that relies on true CT principles rather than diffuse tomography. The in-house built optical system rotates around the sample while Raman spectra are measured from different angles by multiplexing on the 2D CCD. Forward scattered photons are largely in the far-field to reject diffuse photons. We show that R-CT provides both, spatial and molecular information in diffusely scattering phantoms. Data reconstruction is performed using backprojection and multivariate regression analysis to provide a 3D chemical contrast. We finally present preliminary results on rodent limbs. The R-CT technique developed offers a potential technique to improve the characterisation of tissues and drug efficiency in rodent limbs at the molecular level as it offers morphological information that is not available in conventional transmission Raman spectroscopy.

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Citation Download Citation

Elzbieta Stepula, Anders R. Walther, Christine K. Dam, Magnus Jensen, Martin A. B. Hedegaard, and Mads S. Bergholt "Development of forward-scattered Raman tomography for monitoring treatment of rheumatoid arthritis in rodents", Proc. SPIE PC11952, Multimodal Biomedical Imaging XVII, PC119520B (7 March 2022); https://doi.org/10.1117/12.2609556

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KEYWORDS

Arthritis

Raman spectroscopy

Tomography

Chemical analysis

Photons

Tissues

X-ray computed tomography

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