Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the low imaging speed and the accompanying photodamage risk hinder further clinical applications. To reduce these adverse effects, in this study, we constructed a new generation of simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy, featuring high-speed, multimodal imaging, monitorable-photodamage, and tunable ultrashort pulses. By using birefringent photonic crystal fiber and a pulse shaper, this system has the ability to allow users to independently adjust repetition rate, pulse width, and average power without overlapping interference, and can realize multiphoton generation in each channel from a single excitation pulse. These outstanding advantages represent a powerful and user-friendly imaging platform.
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