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Actin, as microfilaments in the cytoskeleton, is essential biological structure and mechanical properties for cell migration and division. These processes require new probes for visualization of actin. Fluorescent labeling as a traditional method accompanies photo bleaching and formation of free radicals that are harmful for live cells, resulting to hardly find a balance between more signals of observation and less light exposure. Here, we present new nanoparticle probes for continuous visualization of actin. We demonstrate continuous imaging of different cell division phases to reveal actin biological and mechanical properties.
Di Zu,Xintao Zhao,Guangjie Cui,Zhijia Zhang, andSomin Eunice Lee
"Live cell probes for continuous visualization of actin dynamics", Proc. SPIE PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV, PC119790D (4 March 2022); https://doi.org/10.1117/12.2610126
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Di Zu, Xintao Zhao, Guangjie Cui, Zhijia Zhang, Somin Eunice Lee, "Live cell probes for continuous visualization of actin dynamics," Proc. SPIE PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV, PC119790D (4 March 2022); https://doi.org/10.1117/12.2610126