Development of an ACE-2 biosensor for point-of-need saliva diagnostics and surveillance of SARS-CoV-2

Geisianny Moreira; Lisseth Casso Hartmann; Shoumen P.A. Datta; Eric S. McLamore; Diana Vanegas

doi:10.1117/12.2622984

8 June 2022 Development of an ACE-2 biosensor for point-of-need saliva diagnostics and surveillance of SARS-CoV-2

Geisianny Moreira, Lisseth Casso Hartmann, Shoumen P.A. Datta, Eric S. McLamore, Diana Vanegas

Proceedings Volume PC12123, Smart Biomedical and Physiological Sensor Technology XIV; PC1212303 (2022) https://doi.org/10.1117/12.2622984
Event: SPIE Defense + Commercial Sensing, 2022, Orlando, Florida, United States

Abstract

The high transmission rate and fast mutation capacity of SARS-CoV-2 requires development of low-cost/widely deployable biosensor technologies. Here, we describe the development of an enzymatic biosensor to monitor SARS-CoV-2 and its emerging variants in human saliva based. Biotinylated angiotensin converting enzyme (ACE2) was immobilized on streptavidin-coated fiber optic probes and gold chips for biolayer interferometry (BLI) and surface plasmon resonance (SPR), respectively. Kinetics of ACE2-virion binding for wild-type, Delta, UK, and South Africa variants were analyzed and compared for informing the development of a mobile detection system.

Conference Presentation

Citation Download Citation

Geisianny Moreira, Lisseth Casso Hartmann, Shoumen P.A. Datta, Eric S. McLamore, and Diana Vanegas "Development of an ACE-2 biosensor for point-of-need saliva diagnostics and surveillance of SARS-CoV-2", Proc. SPIE PC12123, Smart Biomedical and Physiological Sensor Technology XIV, PC1212303 (8 June 2022); https://doi.org/10.1117/12.2622984

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