The high transmission rate and fast mutation capacity of SARS-CoV-2 requires development of low-cost/widely deployable biosensor technologies. Here, we describe the development of an enzymatic biosensor to monitor SARS-CoV-2 and its emerging variants in human saliva based. Biotinylated angiotensin converting enzyme (ACE2) was immobilized on streptavidin-coated fiber optic probes and gold chips for biolayer interferometry (BLI) and surface plasmon resonance (SPR), respectively. Kinetics of ACE2-virion binding for wild-type, Delta, UK, and South Africa variants were analyzed and compared for informing the development of a mobile detection system.
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