Whole-brain fMRI combined with cortex-wide optogenetics

Seong-Gi Kim; Seonghoon Kim; Hyun Seok Moon; Myunghwan Choi

doi:10.1117/12.2661804

17 March 2023 Whole-brain fMRI combined with cortex-wide optogenetics

Seong-Gi Kim, Seonghoon Kim, Hyun Seok Moon, Myunghwan Choi

Proceedings Volume PC12366, Optogenetics and Optical Manipulation 2023; PC1236602 (2023) https://doi.org/10.1117/12.2661804
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Functional Magnetic Resonance Imaging (fMRI) with optogenetic neural manipulation is a powerful tool that enables brain-wide mapping of functional networks. To achieve flexible manipulation of neural excitation throughout the mouse cortex, we incorporated spatiotemporal programmable optogenetic stimuli generated by a digital micromirror device into an MR scanner via an optical fiber bundle. This approach offered versatility in space and time in planning the photostimulation pattern, combined with in situ optical imaging and cell-type or circuit-specific genetic targeting in individual mice. Brain-wide functional connectivity was successfully obtained by fMRI with optogenetic stimulation of atlas-based cortical regions. fMRI combined with flexible optogenetics opens a new path to investigate dynamic changes in functional brain states in the same animal.

Conference Presentation

Citation Download Citation

Seong-Gi Kim, Seonghoon Kim, Hyun Seok Moon, and Myunghwan Choi "Whole-brain fMRI combined with cortex-wide optogenetics", Proc. SPIE PC12366, Optogenetics and Optical Manipulation 2023, PC1236602 (17 March 2023); https://doi.org/10.1117/12.2661804

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