A multimodal approach for margin assessment combines fast, cross-sectional imaging using 1050 nm spectral-domain optical coherence tomography (SD-OCT), offering sensitivity to highly-scattering malignant tissues, with complementary sub-cellular resolution and molecular specificity provided by ultraviolet photoacoustic remote sensing virtual histology. The interferometric synthetic aperture microscopy (ISAM) extension to OCT was implemented to achieve spatially-invariant lateral resolution, reducing the resolution disparity between each modality by avoiding numerical aperture trade-offs. System validation included carbon fiber and TiO2 particle phantom imaging, and ex vivo biological imaging was demonstrated in fresh, thick murine tissues, and benign and malignant human breast samples.
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