Subcellular mechano-microscopy for mechanobiology of 3D cell spheroid cultures

Alireza Mowla; Brendan Kennedy; Matt Hepburn; Jiayue Li; Yu Suk Choi; Samuel Maher; Danielle Vahala

doi:10.1117/12.2647323

9 March 2023 Subcellular mechano-microscopy for mechanobiology of 3D cell spheroid cultures

Alireza Mowla, Brendan Kennedy, Matt Hepburn, Jiayue Li, Yu Suk Choi, Samuel Maher, Danielle Vahala

Proceedings Volume PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII; PC123671C (2023) https://doi.org/10.1117/12.2647323
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Mechanical properties of cells and extracellular matrix play important roles in the regulation of various biological processes such as gene expression, adhesion, and migration of cells. However, it is challenging to map three-dimensional (3D) elasticity on the micro-scale using existing techniques. We propose subcellular mechano-microscopy, a variant of compression-based optical coherence elastography, for 3D elastography of cell spheroids with isotropic elasticity resolution of 10 µm, over large fields of view covering multiple spheroids. We use the proposed technique to scan multicellular non-metastatic breast cancer cell spheroids embedded in gelatin methacryloyl (GelMA) hydrogels and co-register the micro-elastograms with fluorescence microscopy images.

Conference Presentation

Citation Download Citation

Alireza Mowla, Brendan Kennedy, Matt Hepburn, Jiayue Li, Yu Suk Choi, Samuel Maher, and Danielle Vahala "Subcellular mechano-microscopy for mechanobiology of 3D cell spheroid cultures", Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123671C (9 March 2023); https://doi.org/10.1117/12.2647323

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