Unsupervised extraction of light-evoked outer retina dynamics in rodents with spatial heterogeneity

Huakun Li; Bingyao Tan; Yueming Zhuo; Le Han; Rajeshkumar Mupparapu; Veluchamy A. Barathi; Leopold Schmetterer; Tong Ling

doi:10.1117/12.3003459

13 March 2024 Unsupervised extraction of light-evoked outer retina dynamics in rodents with spatial heterogeneity

Huakun Li, Bingyao Tan, Yueming Zhuo, Le Han, Rajeshkumar Mupparapu, Veluchamy A. Barathi, Leopold Schmetterer, Tong Ling

Author Affiliations +

Proceedings Volume PC12824, Ophthalmic Technologies XXXIV; PC128240Y (2024) https://doi.org/10.1117/12.3003459
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

In-vivo imaging of the light-evoked responses of retinal cells in rodents can provide valuable insights into the correlation between optoretinography (ORG) signals and retinal degeneration. However, interpreting outer retina dynamics in rodents is challenging due to the limited resolution of optical coherence tomography, which often results in the superposition of outer retinal layers, such as the rod outer segment (ROS), retinal pigment epithelium (RPE), and Bruch’s membrane, within speckle patterns. Here, we present an automated, unbiased approach for extracting spatially-resolved outer retinal dynamics from complicated speckle patterns. Using this approach, we revealed the light-evoked dynamics of both ROS and RPE in rodents.

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Huakun Li, Bingyao Tan, Yueming Zhuo, Le Han, Rajeshkumar Mupparapu, Veluchamy A. Barathi, Leopold Schmetterer, and Tong Ling "Unsupervised extraction of light-evoked outer retina dynamics in rodents with spatial heterogeneity", Proc. SPIE PC12824, Ophthalmic Technologies XXXIV, PC128240Y (13 March 2024); https://doi.org/10.1117/12.3003459

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