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13 March 2024 Multiphoton microscopy of A. phagocytophilum infection
Alex J. Walsh, Vidhya Shree Ravi, Adela Oliva Chavez
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Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470M (2024) https://doi.org/10.1117/12.3003301
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Anaplasma phagocytophilum is the intracellular bacteria that causes Human Granulocytic Anaplasmosis (HGA). While the skin is known as the entry site for the bacteria, HGA is characterized by systemic infection of A. phagocytophilum, but it is unknown how a localized infection of A. phagocytophilum leads to HGA. Here, we use a multimodal microscopy approach to image collagen and immune cell alterations due to A. phagocytophilum infection. Second-harmonic generation imaging revealed collagen fiber density decreased at the tick bite of infected skin. Additionally, significant differences in autofluorescence lifetime were observed due to A. phagocytophilum infection of human myeloid HL-60 cells.
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Alex J. Walsh, Vidhya Shree Ravi, and Adela Oliva Chavez "Multiphoton microscopy of A. phagocytophilum infection", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470M (13 March 2024); https://doi.org/10.1117/12.3003301
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KEYWORDS
Skin
Multiphoton microscopy
Collagen
Bacteria
Autofluorescence
Diseases and disorders
Multiphoton fluorescence microscopy
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