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13 March 2024 Non-invasive assessment of the developmental potential of embryos using digital holographic microscopy
Kylie R. Dunning, Darren J.X. Chow, George O. Dwapanyin, Tiffany C. Y. Tan, Nicolas S. Dubost, Josephine M. Morizet, Kishan Dholakia
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Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC128540V (2024) https://doi.org/10.1117/12.3001273
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate diagnostic for embryo quality remains elusive in the in vitro fertilization clinic. Determining physical parameters of the embryo may offer key information for this purpose. Here, we demonstrate that digital holographic microscopy (DHM) can rapidly and non-invasively assess the refractive index of mouse embryos. Murine embryos were cultured in either low- or high-lipid containing media and digital holograms recorded at various stages of development. We showed that DHM can detect spatio-temporal changes in refractive index during embryo development that are reflective of its lipid content. As accumulation of intracellular lipid is known to compromise embryo health, DHM may prove beneficial in developing an accurate, non-invasive, multimodal diagnostic.
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Kylie R. Dunning, Darren J.X. Chow, George O. Dwapanyin, Tiffany C. Y. Tan, Nicolas S. Dubost, Josephine M. Morizet, and Kishan Dholakia "Non-invasive assessment of the developmental potential of embryos using digital holographic microscopy", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540V (13 March 2024); https://doi.org/10.1117/12.3001273
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KEYWORDS
Digital holography
Holography
Microscopy
Refractive index
Biopsy
Diagnostics
Fetus
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