Size and refractive index determination of single nanoparticles in nanofluidic optical fibers

Mona Shahsavari; Rienk Nieuwland; Ton G. van Leeuwen; Edwin van der Pol

doi:10.1117/12.3002354

13 March 2024 Size and refractive index determination of single nanoparticles in nanofluidic optical fibers

Mona Shahsavari, Rienk Nieuwland, Ton G. van Leeuwen, Edwin van der Pol

Author Affiliations +

Proceedings Volume PC12858, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XXI; PC1285805 (2024) https://doi.org/10.1117/12.3002354
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Nano-CET (Dispertech) was used to determine the refractive index (RI) of extracellular vesicles (EVs)<100 nm, as it simultaneously measures the trajectory and scattering intensity of particles within a nanofluidic optical fiber. A test sample of outdated erythrocyte-derived EVs was developed for RI determination. Polystyrene beads and silica beads of known sizes were used for calibration and validation. A Python algorithm was developed to track the position and scattering intensity of single particles. The determined RIs of silica beads and hollow organosilica beads (HOBs) matched literature values. Measured RI of EVs was higher (1.63±0.06) than anticipated (RI~1.48), perhaps due to an elevated hemoglobin concentration in outdated erythrocyte EVs.

Conference Presentation

Citation Download Citation

Mona Shahsavari, Rienk Nieuwland, Ton G. van Leeuwen, and Edwin van der Pol "Size and refractive index determination of single nanoparticles in nanofluidic optical fibers", Proc. SPIE PC12858, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XXI, PC1285805 (13 March 2024); https://doi.org/10.1117/12.3002354

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