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18 June 2024 Monitoring dynamic metabolic cell interactions in engineered brain tissue models using label-free, multi-modal two photon excited fluorescence
Irene Georgakoudi, Yang Zhang, Maria T. Savvidou, Olga Liaudanskaya, David L. Kaplan
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Proceedings Volume PC12996, Unconventional Optical Imaging IV; PC129960S (2024) https://doi.org/10.1117/12.3022839
Event: SPIE Photonics Europe, 2024, Strasbourg, France
Abstract
Dynamic metabolic reprogramming among neurons and glial cells can be characterized by label-free two photon excited fluorescence intensity and lifetime (FLIM) imaging of engineered brain tissue models consisting of human neurons, astrocytes, and microglia tri-cultures. Lipofuscin is a significant contributor to the overall fluorescence detected. Its removal is important for accurate recovery of metabolic function metrics. Results reveal the important function of glial cells to reduce oxidative stress in neurons over fourteen weeks of co-culture. Distinct metabolic profiles and dynamic changes are also evident for neurons and glial cells. Our study highlights that engineered brain tissue models in combination with label-free two photon imaging offer an excellent platform for understanding cell-cell interactions and are well-suited to improve understanding of the role of metabolic and mitochondrial dysfunction in neurodegenerative diseases.
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Irene Georgakoudi, Yang Zhang, Maria T. Savvidou, Olga Liaudanskaya, and David L. Kaplan "Monitoring dynamic metabolic cell interactions in engineered brain tissue models using label-free, multi-modal two photon excited fluorescence", Proc. SPIE PC12996, Unconventional Optical Imaging IV, PC129960S (18 June 2024); https://doi.org/10.1117/12.3022839
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KEYWORDS
Brain tissue
Fluorescence
Neurons
Two photon excitation microscopy
Alzheimer disease
Emission wavelengths
Image analysis
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