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Spectral noise causes distorted spectra, shifting the central wavelength and thus reducing the accuracy of surface parameter retrieval. A hybrid method combining mathematical-morphology and wavelet-transform (WB)-based filters was used to remove spectral noise. First, a generalized mathematical-morphology (GM) filter was used to remove large-amplitude noise, and then the processed spectra were smoothed using the WT-based filter to remove small-amplitude noise. The simulated noise spectrum and 76 measured canopy spectra for winter wheat were denoised with three filters: the combination filter (CF), GM, and WT. In the simulated experiments, five evaluation indices were calculated to evaluate the denoising effects. For measured spectra, qualitative analyses were performed based on spectral characteristics. Quantitative evaluations were conducted by deriving various vegetation indices from denoised spectra to retrieve wheat’s biophysical and biochemical parameters. The results indicated that the CF removed both large- and small-amplitude noise efficiently, improving signal-to-noise ratio and peak signal-to-noise ratio of simulated noise spectrum and retrieval accuracy of leaf water content (LWC) significantly. Meanwhile, it better maintained the waveform and smoothness of spectrum, improving the retrieval accuracies of leaf area index and chlorophyll data slightly. The coefficient of determination (R2) of developed model between the modified normalized difference water index and LWC was improved from 0.428 to 0.622 using the CF, 0.555 using the GM, and 0.549 using the WT. The R2 and root mean square error between the measured and retrieval LWC were improved from 0.364 and 0.027 to 0.611 and 0.018 using the CF, whereas the corresponding values were 0.504 and 0.022 for the GM, and 0.478 and 0.023 for the WT.
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