Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy

Katja Schenke-Layland; Iris Riemann; Ulrich A. Stock; Karsten König

doi:10.1117/1.1896966

1 March 2005 Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy

Katja Schenke-Layland, Iris Riemann, Ulrich A. Stock, Karsten König

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Journal of Biomedical Optics, Vol. 10, Issue 2, 024017 (March 2005). https://doi.org/10.1117/1.1896966

Abstract

Multiphoton imaging represents a novel and very promising medical diagnostic technology for the high-resolution analysis of living biological tissues. We performed multiphoton imaging to analyzed structural features of extracellular matrix (ECM) components, e.g., collagen and elastin, of vital pulmonary and aortic heart valves. High-resolution autofluorescence images of collagenous and elastic fibers were demonstrated using multifluorophore, multiphoton excitation at two different wavelengths and optical sectioning, without the requirement of embedding, fixation, or staining. Collagenous structures were selectively imaged by detection of second harmonic generation (SHG). Additionally, routine histology and electron microscopy were integrated to verify the observed results. In comparison with pulmonary tissues, aortic heart valve specimens show very similar matrix formations. The quality of the resulting three-dimensional (3-D) images enabled the differentiation between collagenous and elastic fibers. These experimental results indicate that multiphoton imaging with near-infrared (NIR) femtosecond laser pulses may prove to be a useful tool for the nondestructive monitoring and characterization of cardiovascular structures.

©(2005) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Katja Schenke-Layland, Iris Riemann, Ulrich A. Stock, and Karsten König "Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy," Journal of Biomedical Optics 10(2), 024017 (1 March 2005). https://doi.org/10.1117/1.1896966

Published: 1 March 2005

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KEYWORDS

Tissues

Collagen

Heart

Optical fibers

Second-harmonic generation

Femtosecond phenomena

Near infrared

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