Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells

Veronica Gomez-Godinez; Nicole Wakida; Alexander S. Dvornikov; Kyoko Yokomori; Michael W. Berns

doi:10.1117/1.2717684

1 March 2007 Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells

Veronica Gomez-Godinez, Nicole Wakida, Alexander S. Dvornikov, Kyoko Yokomori, Michael W. Berns

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Journal of Biomedical Optics, Vol. 12, Issue 2, 020505 (March 2007). https://doi.org/10.1117/1.2717684

Abstract

An 800-nm 200-fs laser is used to produce DNA damage in rat kangaroo (PtK1) and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of γH2AX, Nbs1, and Rad50 to the site of irradiation 3 to 30 min following laser exposure. It is concluded that the 200-fs near-infrared laser is an excellent source for the production and study of spatially defined regions of DNA damage.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Veronica Gomez-Godinez, Nicole Wakida, Alexander S. Dvornikov, Kyoko Yokomori, and Michael W. Berns "Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells," Journal of Biomedical Optics 12(2), 020505 (1 March 2007). https://doi.org/10.1117/1.2717684

Published: 1 March 2007

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