Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging

Chaincy Kuo; Olivier Coquoz; Tamara L. Troy; Heng Xu; Bradley W. Rice

doi:10.1117/1.2717898

1 March 2007 Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging

Chaincy Kuo, Olivier Coquoz, Tamara L. Troy, Heng Xu, Bradley W. Rice

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Journal of Biomedical Optics, Vol. 12, Issue 2, 024007 (March 2007). https://doi.org/10.1117/1.2717898

Abstract

A new method is described for obtaining a 3-D reconstruction of a bioluminescent light source distribution inside a living animal subject, from multispectral images of the surface light emission acquired on charge-coupled device (CCD) camera. The method uses the 3-D surface topography of the animal, which is obtained from a structured light illumination technique. The forward model of photon transport is based on the diffusion approximation in homogeneous tissue with a local planar boundary approximation for each mesh element, allowing rapid calculation of the forward Green's function kernel. Absorption and scattering properties of tissue are measured a priori as input to the algorithm. By using multispectral images, 3-D reconstructions of luminescent sources can be derived from images acquired from only a single view. As a demonstration, the reconstruction technique is applied to determine the location and brightness of a source embedded in a homogeneous phantom subject in the shape of a mouse. The technique is then evaluated with real mouse models in which calibrated sources are implanted at known locations within living tissue. Finally, reconstructions are demonstrated in a PC3M-luc (prostate tumor line) metastatic tumor model in nude mice.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Chaincy Kuo, Olivier Coquoz, Tamara L. Troy, Heng Xu, and Bradley W. Rice "Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging," Journal of Biomedical Optics 12(2), 024007 (1 March 2007). https://doi.org/10.1117/1.2717898

Published: 1 March 2007

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