New device for real-time bioluminescence imaging in moving rodents

Emilie Roncali; Mickael Savinaud; Olivier Levrey; Kelly L. Rogers; Serge Maitrejean; Bertrand Tavitian

doi:10.1117/1.2976426

1 September 2008 New device for real-time bioluminescence imaging in moving rodents

Emilie Roncali, Mickael Savinaud, Olivier Levrey, Kelly L. Rogers, Serge Maitrejean, Bertrand Tavitian

Author Affiliations +

Journal of Biomedical Optics, Vol. 13, Issue 5, 054035 (September 2008). https://doi.org/10.1117/1.2976426

Abstract

Bioluminescence imaging (BLI) allows detection of biological functions in genetically modified cells, bacteria, or animals expressing a luciferase (i.e., firefly, Renilla, or aequorin). Given the high sensitivity and minimal toxicity of BLI, in vivo studies on molecular events can be performed noninvasively in living rodents. To date, detection of bioluminescence in living animals has required long exposure times that are incompatible with studies on dynamic signaling pathways or nonanaesthetised freely moving animals. Here we develop an imaging system that allows: 1. bioluminescence to be recorded at a rate of 25 images/s using a third generation intensified charge-coupled device (CCD) camera running in a photon counting mode, and 2. coregistration of a video image from a second CCD camera under infrared lighting. The sensitivity of this instrument permits studies with subsecond temporal resolution in nonanaesthetized and unrestrained mice expressing firefly luciferase and imaging of calcium signaling in transgenic mice expressing green fluorescent protein (GFP) aequorin. This imaging system enables studies on signal transduction, tumor growth, gene expression, or infectious processes in nonanaesthetized and freely moving animals.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Emilie Roncali, Mickael Savinaud, Olivier Levrey, Kelly L. Rogers, Serge Maitrejean, and Bertrand Tavitian "New device for real-time bioluminescence imaging in moving rodents," Journal of Biomedical Optics 13(5), 054035 (1 September 2008). https://doi.org/10.1117/1.2976426

Published: 1 September 2008

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KEYWORDS

Video

Bioluminescence

Signal to noise ratio

Real time imaging

CCD cameras

Imaging systems

Signal detection

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