Macromolecular diffusion in the extracellular matrix measured by fluorescence correlation spectroscopy

Nina Kristine Reitan; Aphirak Juthajan; Tore Lindmo; Catharina de Lange Davies

doi:10.1117/1.2982530

1 September 2008 Macromolecular diffusion in the extracellular matrix measured by fluorescence correlation spectroscopy

Nina Kristine Reitan, Aphirak Juthajan, Tore Lindmo, Catharina de Lange Davies

Author Affiliations +

Journal of Biomedical Optics, Vol. 13, Issue 5, 054040 (September 2008). https://doi.org/10.1117/1.2982530

Abstract

Diffusion of therapeutic macromolecules through the extracellular matrix of tumor tissue is a crucial step in drug delivery. We use fluorescence correlation spectroscopy (FCS) to measure diffusion of IgG (150 kDa) and dextrans (155 kDa and 2 MDa) in solution, 5% gelatin hydrogel, and multicellular spheroids. Gel and spheroids are used as model systems for the extracellular matrix. The diffusion depends on the complexity of the environment, as well as on the size and structural shape of the diffusing molecules. The results based on one-photon FCS are in good agreement with diffusion coefficients obtained with two-photon fluorescence recovery after photobleaching (FRAP) using the same microscope (Zeiss LSM510 META/Confocor2). However, FCS reveals anomalous or multicomponent diffusion in gel and spheroids, which are not resolvable with FRAP. This study demonstrates that one-photon FCS can be used to study the extracellular transport of macromolecules in tumor tissue, and that FCS provides additional information about diffusion properties compared to FRAP.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Nina Kristine Reitan, Aphirak Juthajan, Tore Lindmo, and Catharina de Lange Davies "Macromolecular diffusion in the extracellular matrix measured by fluorescence correlation spectroscopy," Journal of Biomedical Optics 13(5), 054040 (1 September 2008). https://doi.org/10.1117/1.2982530

Published: 1 September 2008

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 34 scholarly publications.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Diffusion

Fluorescence correlation spectroscopy

Systems modeling

Molecules

Data modeling

Tissues

Macromolecules

Show All Keywords

RELATED CONTENT

Parameter estimation and analysis model selections in fluorescence correlation spectroscopy
Proceedings of SPIE (October 31 2016)

Identical particle model on biophoton emission
Proceedings of SPIE (September 23 1996)

Principles and applications of fluorescence lifetime correlation spectroscopy
Proceedings of SPIE (May 18 2009)

Tumor vascular volume determines photosensitizer uptake in MATLyLu prostate tumor...
Proceedings of SPIE (February 27 2007)

Application of fluorescence correlation spectroscopy for drug delivery to tumor...
Proceedings of SPIE (December 18 2000)

Determination of the confocal volume for quantitative fluorescence correlation spectroscopy
Proceedings of SPIE (July 12 2007)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years