High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells

Céline C. B. Boutin; Yann Roche; Christine Millot; Regis Deturche; Pascal Royer; Michel Manfait; Jérôme Plain; Pierre Jeannesson; Jean-Marc Millot; Rodolphe Jaffiol

doi:10.1117/1.3155518

1 May 2009 High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells

Céline C. B. Boutin, Yann Roche, Christine Millot, Regis Deturche, Pascal Royer, Michel Manfait, Jérôme Plain, Pierre Jeannesson, Jean-Marc Millot, Rodolphe Jaffiol

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Journal of Biomedical Optics, Vol. 14, Issue 3, 034030 (May 2009). https://doi.org/10.1117/1.3155518

Abstract

Diffusion-time distribution analysis (DDA) has been used to explore the plasma membrane fluidity of multidrug-resistant cancer cells (LR73 carcinoma cells) and also to characterize the influence of various membrane agents present in the extracellular medium. DDA is a recent single-molecule technique, based on fluorescence correlation spectroscopy (FCS), well suited to retrieve local organization of cell membrane. The method was conducted on a large number of living cells, which enabled us to get a detailed overview of plasma membrane microviscosity, and plasma membrane micro-organization, between the cells of the same line. Thus, we clearly reveal the higher heterogeneity of plasma membrane in multidrug-resistant cancer cells in comparison with the nonresistant ones (denoted sensitive cells). We also display distinct modifications related to a membrane fluidity modulator, benzyl alcohol, and two revertants of multidrug resistance, verapamil and cyclosporin-A. A relation between the distribution of the diffusion-time values and the modification of membrane lateral heterogeneities is proposed.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Céline C. B. Boutin, Yann Roche, Christine Millot, Regis Deturche, Pascal Royer, Michel Manfait, Jérôme Plain, Pierre Jeannesson, Jean-Marc Millot, and Rodolphe Jaffiol "High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells," Journal of Biomedical Optics 14(3), 034030 (1 May 2009). https://doi.org/10.1117/1.3155518

Published: 1 May 2009

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