In vivo harmonic generation biopsy of human skin

Szu-Yu Chen; Hai-Yin Wu; Chi-Kuang Sun

doi:10.1117/1.3269676

1 November 2009 In vivo harmonic generation biopsy of human skin

Szu-Yu Chen, Hai-Yin Wu, Chi-Kuang Sun

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Journal of Biomedical Optics, Vol. 14, Issue 6, 060505 (November 2009). https://doi.org/10.1117/1.3269676

Abstract

The ability to in vivo image deep tissues noninvasively with a high resolution is strongly required for optical virtual biopsy. Higher harmonic generation microscopy, combined with second- and third-harmonic generation microscopies, is applied to 17 Asian volunteers' forearm skin. After continuous observation for 30 min, no visible damage was found. Our study proves that harmonic generation biopsy (HGB) is able to satisfy the safety requirement and to provide high penetrability (~300 μm) and submicron resolution all at the same time and is a promising tool for future virtual biopsy of skin diseases. In contrast to a previous study on fixed human skin specimens, a much improved penetrability and much reduced resolution-degradation versus depth are found in this in vivo examination.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Szu-Yu Chen, Hai-Yin Wu, and Chi-Kuang Sun "In vivo harmonic generation biopsy of human skin," Journal of Biomedical Optics 14(6), 060505 (1 November 2009). https://doi.org/10.1117/1.3269676

Published: 1 November 2009

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