Three-dimensional reconstruction in free-space whole-body fluorescence tomography of mice using optically reconstructed surface and atlas anatomy

Xiaofeng Zhang; Cristian Tudorel Badea; G. Allan Johnson

doi:10.1117/1.3258836

1 November 2009 Three-dimensional reconstruction in free-space whole-body fluorescence tomography of mice using optically reconstructed surface and atlas anatomy

Xiaofeng Zhang, Cristian Tudorel Badea, G. Allan Johnson

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Journal of Biomedical Optics, Vol. 14, Issue 6, 064010 (November 2009). https://doi.org/10.1117/1.3258836

Abstract

We present a 3-D image reconstruction method for free-space fluorescence tomography of mice using hybrid anatomical prior information. Specifically, we use an optically reconstructed surface of the experimental animal and a digital mouse atlas to approximate the anatomy of the animal as structural priors to assist image reconstruction. Experiments are carried out on a cadaver of a nude mouse with a fluorescent inclusion (2.4-mm-diam cylinder) implanted in the chest cavity. Tomographic fluorescence images are reconstructed using an iterative algorithm based on a finite element method. Coregistration of the fluorescence reconstruction and micro-CT (computed tomography) data acquired afterward show good localization accuracy (localization error 1.2±0.6 mm). Using the optically reconstructed surface, but without the atlas anatomy, image reconstruction fails to show the fluorescent inclusion correctly. The method demonstrates the utility of anatomical priors in support of free-space fluorescence tomography.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Xiaofeng Zhang, Cristian Tudorel Badea, and G. Allan Johnson "Three-dimensional reconstruction in free-space whole-body fluorescence tomography of mice using optically reconstructed surface and atlas anatomy," Journal of Biomedical Optics 14(6), 064010 (1 November 2009). https://doi.org/10.1117/1.3258836

Published: 1 November 2009

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Cited by 35 scholarly publications and 2 patents.

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KEYWORDS

Luminescence

Reconstruction algorithms

Data acquisition

Cameras

Fluorescence tomography

Chest

Image restoration

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