Analysis of bacterial chemotactic response using dynamic laser speckle

Silvia Murialdo; Gonzalo Hernán Sendra; Lucia Passoni; Ricardo Arizaga; Jorge Froilán Gonzalez; Héctor Jorge Rabal; Marcelo Trivi

doi:10.1117/1.3262608

1 November 2009 Analysis of bacterial chemotactic response using dynamic laser speckle

Silvia Murialdo, Gonzalo Hernán Sendra, Lucia Passoni, Ricardo Arizaga, Jorge Froilán Gonzalez, Héctor Jorge Rabal, Marcelo Trivi

Author Affiliations +

Journal of Biomedical Optics, Vol. 14, Issue 6, 064015 (November 2009). https://doi.org/10.1117/1.3262608

Abstract

Chemotaxis has a meaningful role in several fields, such as microbial physiology, medicine and biotechnology. We present a new application of dynamic laser speckle (or biospeckle) to detect different degrees of bacterial motility during chemotactic response experiments. Encouraging results showed different bacterial dynamic responses due to differences in the hardness of the support in the swarming plates. We compare this method to a conventional technique that uses white light. Both methods showed to be analogous and, in some cases, complementary. The results suggest that biospeckle processed images can be used as an alternative method to evaluate bacterial chemotactic response and can supply additional information about the bacterial motility in different areas of the swarm plate assay that might be useful for biological analysis.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Silvia Murialdo, Gonzalo Hernán Sendra, Lucia Passoni, Ricardo Arizaga, Jorge Froilán Gonzalez, Héctor Jorge Rabal, and Marcelo Trivi "Analysis of bacterial chemotactic response using dynamic laser speckle," Journal of Biomedical Optics 14(6), 064015 (1 November 2009). https://doi.org/10.1117/1.3262608

Published: 1 November 2009

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KEYWORDS

Speckle

Bacteria

Biological research

Image processing

Lanthanum

Signal processing

Cameras

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