Waveguide evanescent field fluorescence microscopy: high contrast imaging of a domain forming mixed lipid Langmuir-Blodgett monolayer mimicking lung surfactant

Abdollah Hassanzadeh; Silvia Mittler

doi:10.1117/1.3569095

1 April 2011 Waveguide evanescent field fluorescence microscopy: high contrast imaging of a domain forming mixed lipid Langmuir-Blodgett monolayer mimicking lung surfactant

Abdollah Hassanzadeh, Silvia Mittler

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Journal of Biomedical Optics, Vol. 16, Issue 4, 046022 (April 2011). https://doi.org/10.1117/1.3569095

Abstract

Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir-Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were fabricated on waveguides using the LB technique and imaged by both WEFF and standard wide field epi-fluorescence microscopy. These two technologies were compared with respect to contrast, photobleaching, and sensitivity. It was found that WEFF microscopy produced images with a much higher contrast, lower photobleaching, and higher sensitivity. In addition, fine structures in the lipidic domains were observed for the first time.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Abdollah Hassanzadeh and Silvia Mittler "Waveguide evanescent field fluorescence microscopy: high contrast imaging of a domain forming mixed lipid Langmuir-Blodgett monolayer mimicking lung surfactant," Journal of Biomedical Optics 16(4), 046022 (1 April 2011). https://doi.org/10.1117/1.3569095

Published: 1 April 2011

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