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13 June 2012 Optical phase nanoscopy in red blood cells using low-coherence spectroscopy
Itay Shock, Alexander Barbul, Pinhas Girshovitz, Uri Nevo, Rafi Korenstein, Natan T. Shaked
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Journal of Biomedical Optics, Vol. 17, Issue 10, 101509 (June 2012). https://doi.org/10.1117/1.JBO.17.10.101509
Abstract
We propose a low-coherence spectral-domain phase microscopy (SDPM) system for accurate quantitative phase measurements in red blood cells (RBCs) for the prognosis and monitoring of disease conditions that affect the visco-elastic properties of RBCs. Using the system, we performed time-recordings of cell membrane fluctuations, and compared the nano-scale fluctuation dynamics of healthy and glutaraldehyde-treated RBCs. Glutaraldehyde-treated RBCs possess lower amplitudes of fluctuations, reflecting an increased membrane stiffness. To demonstrate the ability of our system to measure fluctuations of lower amplitudes than those measured by the commonly used holographic phase microscopy techniques, we also constructed wide-field digital interferometry (WFDI) system and compared the performances of both systems. Due to its common-path geometry, the optical-path-delay stability of SDPM was found to be less than 0.3 nm in liquid environment, at least three times better than WFDI under the same conditions. In addition, due to the compactness of SDPM and its inexpensive and robust design, the system possesses a high potential for clinical applications.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Itay Shock, Alexander Barbul, Pinhas Girshovitz, Uri Nevo, Rafi Korenstein, and Natan T. Shaked "Optical phase nanoscopy in red blood cells using low-coherence spectroscopy," Journal of Biomedical Optics 17(10), 101509 (13 June 2012). https://doi.org/10.1117/1.JBO.17.10.101509
Published: 13 June 2012
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Cited by 31 scholarly publications and 4 patents.
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KEYWORDS
Blood
Microscopy
Spectroscopy
Digital holography
Interferometry
Super resolution microscopy
Phase measurement
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