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7 May 2012 Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics
Martí Duocastella, Bo Sun, Craig B. Arnold
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Journal of Biomedical Optics, Vol. 17, Issue 5, 050505 (May 2012). https://doi.org/10.1117/1.JBO.17.5.050505
Abstract
Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Using an ultra-high speed liquid lens, we circumvent this problem by simultaneously acquiring images from multiple focal planes. We demonstrate this method by imaging microparticles and cells flowing in 3-D microfluidic channels.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Martí Duocastella, Bo Sun, and Craig B. Arnold "Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics," Journal of Biomedical Optics 17(5), 050505 (7 May 2012). https://doi.org/10.1117/1.JBO.17.5.050505
Published: 7 May 2012
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Cited by 57 scholarly publications and 8 patents.
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KEYWORDS
3D image processing
Microscopes
Objectives
Microfluidics
3D acquisition
Microscopy
Adaptive optics
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