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9 July 2015 Intraoperative near-infrared fluorescence imaging and spectroscopy identifies residual tumor cells in wounds
David Holt D.V.M., Ashwin B. Parthasarathy, Olugbenga T. Okusanya M.D., Jane Keating M.D., Ollin Venegas, Charuhas Deshpande, Giorgos C. Karakousis, Brian Madajewski, Amy Durham, Shuming Nie, Arjun G. Yodh, Sunil Singhal M.D.
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Journal of Biomedical Optics, Vol. 20, Issue 7, 076002 (July 2015). https://doi.org/10.1117/1.JBO.20.7.076002
Abstract
Surgery is the most effective method to cure patients with solid tumors, and 50% of all cancer patients undergo resection. Local recurrences are due to tumor cells remaining in the wound, thus we explore near-infrared (NIR) fluorescence spectroscopy and imaging to identify residual cancer cells after surgery. Fifteen canines and two human patients with spontaneously occurring sarcomas underwent intraoperative imaging. During the operation, the wounds were interrogated with NIR fluorescence imaging and spectroscopy. NIR monitoring identified the presence or absence of residual tumor cells after surgery in 14/15 canines with a mean fluorescence signal-to-background ratio (SBR) of ∼16. Ten animals showed no residual tumor cells in the wound bed (mean SBR<2, P<0.001). None had a local recurrence at <1-year follow-up. In five animals, the mean SBR of the wound was <15, and histopathology confirmed tumor cells in the postsurgical wound in four/five canines. In the human pilot study, neither patient had residual tumor cells in the wound bed, and both remain disease free at <1.5-year follow up. Intraoperative NIR fluorescence imaging and spectroscopy identifies residual tumor cells in surgical wounds. These observations suggest that NIR imaging techniques may improve tumor resection during cancer operations.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2015/$25.00 © 2015 SPIE
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David Holt D.V.M., Ashwin B. Parthasarathy, Olugbenga T. Okusanya M.D., Jane Keating M.D., Ollin Venegas, Charuhas Deshpande, Giorgos C. Karakousis, Brian Madajewski, Amy Durham, Shuming Nie, Arjun G. Yodh, and Sunil Singhal M.D. "Intraoperative near-infrared fluorescence imaging and spectroscopy identifies residual tumor cells in wounds," Journal of Biomedical Optics 20(7), 076002 (9 July 2015). https://doi.org/10.1117/1.JBO.20.7.076002
Published: 9 July 2015
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KEYWORDS
Tumors
Luminescence
Imaging spectroscopy
Near infrared
Tissues
Fluorescence spectroscopy
Spectroscopy
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