Longitudinal study of hemodynamics and dendritic membrane potential changes in the mouse cortex following a soft cranial window installation

Hyejin Park; Nayeon You; Juheon Lee; Minah Suh

doi:10.1117/1.NPh.6.1.015006

25 February 2019 Longitudinal study of hemodynamics and dendritic membrane potential changes in the mouse cortex following a soft cranial window installation

Hyejin Park, Nayeon You, Juheon Lee, Minah Suh

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Neurophotonics, Vol. 6, Issue 1, 015006 (February 2019). https://doi.org/10.1117/1.NPh.6.1.015006

Abstract

The soft cranial window using polydimethylsiloxane allows direct multiple access to neural tissue during long-term monitoring. However, the chronic effects of soft window installation on the brain have not been fully studied. Here, we investigate the long-term effects of soft window installation on sensory-evoked cerebral hemodynamics and neuronal activity. We monitored the brain tissue immunocytohistology for 6 weeks postinstallation. Heightened reactive astrocytic and microglia levels were found at 2 weeks postinstallation. By 6 weeks postinstallation, mice had expression levels similar to those of normal animals. We recorded sensory-evoked hemodynamics of the barrel cortex and LFP during whisker stimulation at these time points. Animals at 6 weeks postinstallation showed stronger hemodynamic responses and focalized barrel mapping than 2-week postoperative mice. LFP recordings of 6-week postoperative mice also showed higher neural activity at the barrel column corresponding to the stimulated whisker. Furthermore, the expression level of interleukin-1β was highly upregulated at 2 weeks postinstallation. When we treated animals postoperatively with minocycline plus N-acetylcystein, a drug-suppressing inflammatory cytokine, these animals did not show declined hemodynamic responses and neuronal activities. This result suggests that neuroinflammation following soft window installation may alter hemodynamic and neuronal responses upon sensory stimulation.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Hyejin Park, Nayeon You, Juheon Lee, and Minah Suh "Longitudinal study of hemodynamics and dendritic membrane potential changes in the mouse cortex following a soft cranial window installation," Neurophotonics 6(1), 015006 (25 February 2019). https://doi.org/10.1117/1.NPh.6.1.015006

Received: 10 October 2018; Accepted: 25 January 2019; Published: 25 February 2019

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