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13 March 2024 Three-dimensional visualization of cerebrovascular metrics in 3xTg mouse models of Alzheimer's disease
Mio Jiang, Danny Xie, Christian Crouzet, Bernard Choi
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Proceedings Volume PC12828, Neural Imaging and Sensing 2024; PC128280G (2024) https://doi.org/10.1117/12.3003103
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Altered brain vasculature plays a crucial role in neurologic disorders, including Alzheimer's Disease (AD). Recent evidence suggests that vascular changes precede the characteristic cognitive decline in AD, highlighting the potential of cerebrovascular biomarkers as preclinical tools. However, there is a need for improved visualization techniques. Our previous work developed a protocol to visualize brain vasculature in three dimensions using perfusion-based labeling, a modified iDISCO+ clearing protocol, and confocal imaging. Focusing on the 3xTg mouse model, an established AD pathogenesis model, we compare cerebrovascular biomarkers between wild-type and 3xTg mice to understand associated alterations. We use custom MATLAB algorithms and open-source neuron tracing software, neuTube, to segment vessels in fluorescence images and quantify metrics such as diameter, density, and tortuosity. Quantifying these metrics shows promise in uncovering cerebrovascular mechanisms contributing to AD and neurologic conditions, enabling early detection and targeted interventions.
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Mio Jiang, Danny Xie, Christian Crouzet, and Bernard Choi "Three-dimensional visualization of cerebrovascular metrics in 3xTg mouse models of Alzheimer's disease", Proc. SPIE PC12828, Neural Imaging and Sensing 2024, PC128280G (13 March 2024); https://doi.org/10.1117/12.3003103
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KEYWORDS
3D modeling
Alzheimer disease
Mouse models
Visualization
Animal model studies
Brain
Biomedical optics
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