Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers

Björn Kemper; Luisa Pohl; Mathias Kaiser; Eva Döpker; Jürgen Schnekenburger; Steffi Ketelhut

doi:10.1117/12.2509242

4 March 2019 Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers

Björn Kemper, Luisa Pohl, Mathias Kaiser, Eva Döpker, Jürgen Schnekenburger, Steffi Ketelhut

Author Affiliations +

Proceedings Volume 10887, Quantitative Phase Imaging V; 108871M (2019) https://doi.org/10.1117/12.2509242
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

We have explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images for example of digital holographic microscopy (DHM), a variant of quantitative phase microscopy (QPM). The applicability of the proposed numerical procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. The achieved results show that histogram-based evaluation of quantitative phase images allows a highly reliable detection and continuous observation of global cellular morphology changes in confluent cell layers.

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Björn Kemper, Luisa Pohl, Mathias Kaiser, Eva Döpker, Jürgen Schnekenburger, and Steffi Ketelhut "Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers", Proc. SPIE 10887, Quantitative Phase Imaging V, 108871M (4 March 2019); https://doi.org/10.1117/12.2509242

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