New approaches for the analysis of confluent cell layers with quantitative phase digital holographic microscopy

L. Pohl; M. Kaiser; S. Ketelhut; S. Pereira; F. Goycoolea; Björn Kemper

doi:10.1117/12.2212523

9 March 2016 New approaches for the analysis of confluent cell layers with quantitative phase digital holographic microscopy

L. Pohl, M. Kaiser, S. Ketelhut, S. Pereira, F. Goycoolea, Björn Kemper

Author Affiliations +

Proceedings Volume 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII; 97130D (2016) https://doi.org/10.1117/12.2212523
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Digital holographic microscopy (DHM) enables high resolution non-destructive inspection of technical surfaces and minimally-invasive label-free live cell imaging. However, the analysis of confluent cell layers represents a challenge as quantitative DHM phase images in this case do not provide sufficient information for image segmentation, determination of the cellular dry mass or calculation of the cell thickness. We present novel strategies for the analysis of confluent cell layers with quantitative DHM phase contrast utilizing a histogram based-evaluation procedure. The applicability of our approach is illustrated by quantification of drug induced cell morphology changes and it is shown that the method is capable to quantify reliable global morphology changes of confluent cell layers.

Citation Download Citation

L. Pohl, M. Kaiser, S. Ketelhut, S. Pereira, F. Goycoolea, and Björn Kemper "New approaches for the analysis of confluent cell layers with quantitative phase digital holographic microscopy", Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 97130D (9 March 2016); https://doi.org/10.1117/12.2212523

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