Problems And Caveats Associated With The Determination Of Protein Conformation By FT-IR Spectroscopy

H. H. Mantsch; W. K. Surewicz; A. Muga; D J Moffatt; H L. Casal

doi:10.1117/12.969611

1 December 1989 Problems And Caveats Associated With The Determination Of Protein Conformation By FT-IR Spectroscopy

H. H. Mantsch, W. K. Surewicz, A. Muga, D J Moffatt, H L. Casal

Proceedings Volume 1145, 7th Intl Conf on Fourier Transform Spectroscopy; (1989) https://doi.org/10.1117/12.969611
Event: Seventh International Conference on Fourier and Computerized Infrared Spectroscopy, 1989, Fairfax, VA, United States

Abstract

Infrared spectroscopy is being used increasingly to study the conformational structure of proteins and polypeptides in aqueous solution. The methodology generally used for the infrared spectroscopic analysis of protein secondary structure is based on three steps: 1. Separation of the overlapping amide I (amide C = O stretching) component bands via band-narrowing procedures such as Fourier self-deconvolution or derivation. 2. Assignment of the resolved component bands, based on previously established spectra-structure correlations, to different secondary structure elements, i.e., alpha helices, beta-sheets, turns or non-ordered conformations. 3. Extraction of quantitative information on protein secondary structure from analysis of amide I band profiles by curve fitting. Each of these three steps has potential sources of error which have to be recognized to prevent fallacious interpretations.

Citation Download Citation

H. H. Mantsch, W. K. Surewicz, A. Muga, D J Moffatt, and H L. Casal "Problems And Caveats Associated With The Determination Of Protein Conformation By FT-IR Spectroscopy", Proc. SPIE 1145, 7th Intl Conf on Fourier Transform Spectroscopy, (1 December 1989); https://doi.org/10.1117/12.969611

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available