Light-sheet microscopy (LSM) has emerged as the technique of choice for many biologists imaging large cleared tissues due to its speed and optical efficiency, which make it possible to generate massive datasets of large specimens at high resolution. Here, we build on several recent innovations in LSM to present a non-orthogonal dual-objective (NODO) LSM system with axial sweeping in an open-top configuration. This system is specifically designed to image large cleared brain tissues, such as for axonal connectomics, and provides subcellular resolution (0.3 µm lateral, 2 µm axial) of large cleared samples up to 8 mm thick.
|